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Anticancer Res 2001 Nov-Dec;21(6A):3903-8
Strong antioxidant activity of ellagic acid in mammalian cells in
vitro revealed by the comet assay. Festa F, Aglitti T, Duranti G, Ricordy R, Perticone P, Cozzi R.
Dipartimento di Biologia, Universita degli Studi Roma TRE, Italy.
Oxidative stress due to oxygen and various radical species is associated
with the induction of DNA single- and double-strand breaks and is considered
to be a first step in several human degenerative diseases, cancer and
ageing. Naturally occurring antioxidants are being extensively analysed for
their ability to protect DNA against such injury. We studied three naturally
occuring compounds,Ascorbic Acid, Melatonin and Ellagic acid, for their
ability to modulate DNA damage produced by two strong radical oxygen
inducers (H2O2 and Bleomycin) in cultured CHO cells. The alkaline Comet
assay was used to measure DNA damage and a cytofluorimetric analysis was
performed to reveal the intracellular oxidative species. The data showed a
marked reduction of H2O2- and Bleomycin-induced DNA damage exerted by
Ellagic Acid. On the contrary Ascorbic acid and Melatonin appeared to induce
a slight increase in DNA damage per se. In combined treatments, they caused
a slight reduction of H2O2-induced damage, but they did not efficiently
modulate the Bleomycin-induced one. The Dichlorofluorescein diacetate
(DCFH-DA)cytofluorimetric test confirmed the strong scavenging action
exerted by Ellagic Acid.
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